Regulatory

Part:BBa_I735009

Designed by: Giovanni Russo   Group: iGEM07_Naples   (2007-10-19)

ORE2X oleate response element

Ore is a consensus sequence localized in some promoters that respond to oleic acid concentration. Its sequence is CGGN3TNA/(R)N8-12CCG. In this case we have 2 ore consensus sequences. We digested ORE2X with XbaI and SpeI restriction enzymes and then we cloned it in pSB1A3 plasmid.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 98
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//rnap/miscellaneous
//direction
//chassis/miscellaneous
//promoter
//regulation/unknown
Parameters
negative_regulators-1
positive_regulators-1